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BspQI BspQI限制性内切酶
BspQ I is a type IIS restriction enzyme that creates linearized, scarless templates from plasmid DNA that are suitable for in vitro transcription. BspQ I (10 U/μl) and 10X BspQ I Buffer are included.
Product Information
| Source | E.coli |
Restriction Enzyme Cutting Site | 5’…GCTCTTC(N)1↓…3’ |
3’…CGAGAAG(N)4↑…5’ | |
Storage Buffer | 20 mM Tris-HCl, 500 mM KCl, 1 mM DTT, 0.1 mM EDTA, 500 µg/ml HSA, 50% Glycerol, 0.1% Triton X-100, pH 7 |
Enzyme Activity Definition | In a total reaction of 50 μl, at 50°C, the amount of enzyme required to digest 1 μg of λDNA in 1 h is defined as 1 activity unit (U) . |
Transportation/Storage Condition | Dry ice transport, store at -20 ± 5 °C. Avoid repeated freeze-thaw cycles |
Product Applications | Molecular cloning, restriction site mapping, genotyping, SNPs, etc. |
Specifications
| Components | Cat. #/Size | |
| BSP-BE101-A (1000 U) | BSP-BE101-B(10 kU) | |
| BspQI(10 U/μl) | BSP-DL101-1000U(100 μl) | BSP-DL101-10KU(1 ml) |
| 10×BspQI Reaction Buffer | BSP-DL101-80(800 μl) | BSP-DL101-800(8 ml) |
Cautions
| (1) BspQI is insensitive to Dam, Dcm, and CpG methylation. |
| (2) Before the enzymatic digestion reaction, try to ensure that the DNA does not contain impurities such as phenol, chloroform, alcohol, EDTA, etc., so as not to affect the enzymatic digestion effect. |
| (3) The supercoiled plasmid may hide the digestion site of BspQI and affect the digestion effect of BspQI, the enzyme amount can be increased appropriately or the digestion time can be extended to 1-2 h. |
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