398.50
DNase I (RNase-free) DNase I 无 RNase 替代 NEB M0303S NEB M0303L Promega Z3585 Thermo EN0521
大包装致电或咨询客服
品质保证
顺丰发货
专票13
选择 包装规格
  • 产品详情
  • 产品参数
  • 产品评论


DNase I (RNase-free) DNase I 无 RNase Deoxyribonuclease I 脱氧核糖核酸酶I 替代 NEB M0303S NEB M0303L Promega Z3585 Thermo EN0521


DNase I.png

DNase I digests DNA efficiently

Fig.1 Add 1 μg DNA and different activity units of DNaseI in a total reaction volume of 10 μl. Compared with competitor 1 and 2, DNase I of kactus has a stronger digestive effect.


Product Information


Source

Recombinant enzyme, from a non-animal host

Storage Buffer

10mM Tris-HCl (pH7.6)
2mM CaCl2
50% Glycerol

Transportation/ Storage Method

Shipped on dry ice, stored at -20°C, valid for one year, in order to avoid repeated freezing and thawing,   it is recommended to repack immediately after receiving

Enzyme Activity Definition

One unit (1U):   The amount of enzyme required to completely degrade 1 μg of pBR322 plasmid DNA within 10 minutes at 37°C.

Product application

1. Preparation of DNA-free RNA samples.
2. Remove the template DNA after in vitro transcription.
3. Remove contaminating genomic DNA from RNA samples.
4. Used in conjunction with DNA polymerase I for DNA labeling by incision   translation.
5. Used in Footprinting method to analyze DNA-protein interaction.
6. DNA fragment library construction in the presence of Mn2.


Product Components


Product Components

Cat. No. /Size

DNI-DL001-B (1000U)

DNI-DL001-C (5000U)

DNase I (RNase-free, 4U/μl)

250μl

1.25ml


Other Notes


1. Put this product on ice for operation, and store it at -20°C after use.

2. In RNA extraction experiments, it is recommended to add an appropriate amount of RNase Inhibitor to prevent RNA degradation.

3. Metal ion chelators, 0.1% SDS, DTT and mercaptoethanol have significant inhibitory effects on this product.

4.Please wear lab clothes and disposable gloves during operation.



提示:本产品仅供工业应用或科学研究使用!


大包装敬请您电话咨询:021-5161 9931,或加微信 136 5169 8516,小递全心全意为您服务!


小递价格
398.50
所属品牌
DiLab
来源
non-animal host
纯度
4U/μl,以每批次为准
首页
客服
购物车
加入购物车
立即购买