- 产品详情
- 产品参数
- 产品评论
XbaI XbaI 限制性内切酶 替代NEB R0145V NEB R0145S NEB R0145T NEB R0145L NEB R0145M
XbaI restriction endonuclease that recognizes the sequence T^CTAG_A.
Product Information
| Source | E.coli |
Restriction Enzyme Cutting Site | 5’…T↓CTAGA…3’ |
3’…AGATC↑T…5’ | |
Storage Buffer | 10 mM Tris-HCl, 50 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 200 μg/ml HSA, 50% Glycerol, pH 7.4 |
Enzyme Activity Definition | One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA (dam-/HindIII digest) in 1 hour at 37°C in a total reaction volume of 50 µl. |
Transportation/Storage Condition | Dry ice transport, store at -20 ± 5 °C. Avoid repeated freeze-thaw cycles |
Product Applications | Molecular cloning, restriction digestion, genotyping, Southern blotting, SNPs, etc |
Specifications
| Components | Cat. #/Size | |
| XBA-DL101-2000 U | XBA-DL101-20 KU | |
| XbaI (20 U/μl) | XBA-DL101-E (100 μl) | XBA-DL101-E (1ml) |
| 10×Cut Reaction Buffer | XBA-DL001-B7.5 (750 μl) | XBA-DL001-B75 (7.5ml) |
Cautions
| (1) XbaI is affected by Dam methylation and sequences may overlap, blocking cleavage. |
提示：本产品仅供工业应用或科学研究使用！
大包装敬请您电话咨询：021-5161 9931，或加微信 136 5169 8516，小递全心全意为您服务！