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SapI SapI限制性内切酶 替代NEB R0569S NEB R0569L
SapI is a restriction endonuclease that recognizes the sequence GCTCTTCN^NNN_.. BspQ I (10 U/μl) and 10X BspQ I Buffer are included.
Product Information
| Source | E.coli |
Restriction Enzyme Cutting Site | 5’…GCTCTTC(N)1↓…3’ |
3’…CGAGAAG(N)4↑…5’ | |
Storage Buffer | 10 mM Tris-HCl, 300 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 500 μg/ml HSA, 50% Glycerol, pH 7.4 |
Enzyme Activity Definition | One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl. |
Transportation/Storage Condition | Dry ice transport, store at -20 ± 5 °C. Avoid repeated freeze-thaw cycles |
Product Applications | Molecular cloning, restriction site mapping, genotyping, SNPs, etc. |
Specifications
| Components | Cat. #/Size | |
| SAP-DL101-1000 U | SAP-DL101-1000 U | |
| SapI (10 U/μl) | SAP-DL101-E (100 μl) | SAP-DL101-E (1ml) |
| 10×Cut Reaction Buffer | CUT-DL001-B8 (800 μl) | CUT-DL001-B80 (8ml) |
Assay Data
Fig. 1 The digestion efficiency of DiLab SapI is greater than competitor, and basically equivalent to the BspQI digestion efficiency of the competitor. 1 μg plasmid was added to 1 μl SapI/BspQI (lane NC was control, lane 1 - 4 diluted at 2 times gradient, lane 1 was stock solution) in a 15 min at 37°C/50°C with a total reaction volume of 50 μl.
Fig. 2 1 μg plasmid was added to 1 μl SapI (lane NC was control, lane 1 - 5 diluted at 2 times gradient, lane 1 was stock solution) in a 16 h at 37°C with a total reaction volume of 50 μl.
Cautions
| (1) BspQI is insensitive to Dam, Dcm, and CpG methylation. |
| (2) Before the enzymatic digestion reaction, try to ensure that the DNA does not contain impurities such as phenol, chloroform, alcohol, EDTA, etc., so as not to affect the enzymatic digestion effect. |
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