- 产品详情
- 产品参数
- 产品评论
Pyrophosphatase Inorganic 焦磷酸酶 无机的 替代NBE M2403S NEB M2403L Thermo EF0221 Thermo EF022SKAFB003 Thermo EF022SKAFB001 Thermo EF022SKAFB002 Thermo EF022SKB003 Thermo EF022SKB006 Thermo EF022SKB008 GMP grade
Inorganic pyrophosphatase effectively increases the yield of mRNA
Fig. 1 The addition of inorganic pyrophosphatase to IVT reactions can effectively increase mRNA yield, lane 1 was without inorganic pyrophosphatase, while lane 2 was added inorganic pyrophosphatase.
Product Information
This product is a yeast-derived inorganic pyrophosphatase (PPase) expressed by recombinant Escherichia coli. It is an enzyme that catalyzes the conversion of one molecule of pyrophosphate into two molecules of phosphate ions, and hydrolyzes inorganic pyrophosphate to form orthophosphate.This is a high-energy reaction. Therefore, this reaction can be coupled to some thermodynamically unfavorable transformations, so as to drive these transformations completely. In molecular biology, it can be used to increase mRNA production in in vitro transcription reactions.
This product is based on the company's unique innovative functional recombinant protein production platform SAMS. After the optimization of the E. coli expression system and purification process, it is produced in accordance with GMP requirements.
| Product Name | T7 RNA Polymerase |
Product Characters | The solution is clear without suspension, precipitation or impurities |
Source | Recombinant E.coli |
Storage Condition | -20±5℃ |
Quality Control
| Activity | 126U/ml |
Purity (SEC-HPLC) | ≥95% |
Nonspecific nuclease residue | negative |
Endonuclease residue | negative |
Exonuclease residue | negative |
RNA enzyme residue | negative |
Protease residue | negative |
Heavy metal residue | <10ppm |
Bacterial endotoxin | <10eu/ml |
Host DNA residue | <100pg/mg |
Host protein residue | <50ng/mg |
Nickel salt residue | <10ppm |
Microbial limit | <1cfu/10ml |
pH | 8.0±0.5 |
Product Components
| Cat. No. | Product Components | Size |
GMP-DL-YE101-100U | Pyrophosphatase, Inorganic(0.1U/μl) | 100U，1ml /vial |
GMP-DL-YE101-800U | 800U，8ml /vial |
Other Notes
(1) The optimum reaction temperature of this product is 25°C, it is active at 16°C-37°C, and the enzyme can be inactivated at 65°C for 10 minutes.
(2) It is recommended to store in aliquots to avoid repeated freezing and thawing.
(3) Please wear lab clothes and disposable gloves during operation.
提示：本产品仅供工业应用或科学研究使用！
大包装敬请您电话咨询：021-5161 9931，或加微信 136 5169 8516，小递全心全意为您服务！