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T7 RNA Polymerase T7 RNA 聚合酶 替代NEB M0251 NEB M0460T Thermo EP0113 Thermo EP0111 Thermo EP0112 Invitrogen 18033100 Invitrogen 18033019 GMP grade
Efficient transcription of mRNA of different lengths
Fig. 1 Three plasmids (lane 1 with poly A tail, lane 2 and 3 without poly A tail) were used as templates, and the reaction was 2 h at 37 °C in the 20 μl system, and the transcription length was 2000 nt, 4000 nt, and 2000 nt, respectively. The above mRNA was not purified.
Product Information
T7 RNA polymerase is the protein encoded by phage T7 DNA expressed by E.coli is a DNA dependent 5 '→ 3' RNA polymerase that highly specifically recognizes the T7 promoter sequence (5 '-taatacgactcactatag*-3'). This product uses single stranded or double stranded DNA containing T7 promoter sequence as template and NTP as substrate to synthesize RNA strand complementary to single stranded or double stranded DNA template strand downstream of promoter.
This product is based on the company's unique innovative functional recombinant protein production platform SAMs. After the optimization of E. coli expression system and purification process, it is produced in accordance with GMP requirements, meeting the product quality standards, and meeting the requirements of mRNA vaccine production.
| Product Name | T7 RNA Polymerase |
Product Characters | The solution is clear without suspension, precipitation or impurities |
Source | An E.coli strain that carries a plasmid encoding the engineered T7 RNA Polymerase gene. |
Storage Condition | -20±5℃ |
Quality Control
| Activity | ≥ 50ku/ml |
Purity (SEC-HPLC) | ≥95% |
Nonspecific nuclease residue | negative |
Endonuclease residue | negative |
Exonuclease residue | negative |
RNA enzyme residue | negative |
Protease residue | negative |
Heavy metal residue | <10ppm |
Bacterial endotoxin | <10eu/ml |
Host DNA residue | <100pg/mg |
Host protein residue | <50ng/mg |
Nickel salt residue | <10ppm |
Microbial limit | <1cfu/10ml |
pH | 7.9±0.5 |
Product Components
| Cat No | Product Components | Size |
GMP-DLP-EE101-50kU | T7 RNA Polymerase | 50kU，1ml/vial |
5×Transcription Buffer | 6ml/vial | |
GMP-DLP-EE101-1MU | T7 RNA Polymerase | 1MU，20ml/vial |
5×Transcription Buffer | 60ml /vial |
Cautions
(1) it is recommended to add DNA template at last to prevent Spermidine in the 5× transcription buffer precipitates the DNA template.
(2) The DNA template is pre cut into flat ends or 5'protruding ends, which is conducive to the effective transcription of specific regions.
(3) In order to avoid the influence of protein and salt ions on the system, it is suggested that the plasmid be purified after linearization and then used as a template for in vitro transcription.
(4) Products should avoid repeated freeze-thawing.
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