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SD208 TGF-βRI (ALK5) inhibitor 627536-09-8
Biological Activity
SD-208 blocka TGF-beta-induced phosphorylation of the receptor-associated Smads, Smad2 and Smad3, and stimulates epithelial-to-mesenchymal transdifferentiation, migration, and invasiveness into Matrigel in vitro.In the murine aortic allograft model, SD-208 effectively reduces the formation of intimal hyperplasia of transplant arteriosclerosis (TA).
Chemical Information
| Molecular Weight | 352.75 |
Formula | C17H10ClFN6 |
CAS Number | 627536-09-8 |
Solubility (25°C) | DMSO 8 mg/mL warmed |
Storage | Powder -20°C 3 years ; 4°C 2 years |
| In solvent -80°C 6 months ; -20°C 1 month |
Protocol (for reference only)
Cell Experiment | |
Cell lines | Murine SMA-560 or human LN-308 glioma cells |
Preparation method | Proliferation. |
Glioma cells were cultured in the absence or presence of SD-208 (1 μmol/L) for 48 hours. The cells were pulsed for the last 24 hours with [methyl-3H]thymidine (0.5 μCi) and harvested (Tomtec, Hamden, CT), and incorporated radioactivity was determined in a liquid scintillation counter (Wallac, Turku, Finland). | |
Concentrations | 1 μM |
Incubation time | 48 h |
Animal Experiment | |
Animal models | SMA-560 cells bearing VM/Dk mice |
Formulation | deionized water |
Dosages | 1 mg/mL |
Administration | p.o. |
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.