- Detail
- Parameters
- Review
Recombinant Trypsin Trypsin Protease Pharmacopoeia grade Trypsin & Cell Culture grade Trypsin 9002-07-7 (Freeze-dried powder)
PRODUCT DESCRIPTION
Recombinant trypsin is a serine protease, which can specifically cleave the C-terminal peptide bond of lysine and arginine. This product is a cell culture-grade genetically engineered enzyme, which does not contain enzyme inhibitors such as DFP, PMSF and TLCK, and has the same activity and specificity as natural trypsin. The activity of this product is inhibited by serine protease inhibitors such as PMSF and TLCK, as well as metal ion chelators such as EDTA, etc.
PRODUCT FEATURES:
Conforms to the standard of Chinese Pharmacopoeia 2020
Animal origin free: Recombinant production, no exogenous virus contamination. No animal-derived material is used during production.
Stable quality: stable and continuous batch production can be guaranteed; There is no difference between product batches and the quality is stable.
High purity: no any other contaminated proteases and non- specific cutting sites.
High Production：Ton-level fermentor employed in production, batch output can be 200g,suitable for industrial customers.
Support customization：Specifications can be customized according to requirements.
UNIT DEFINITION
25 ℃, pH 7.6, reaction system 3.0ml (1cm optical path).
Enzymatic digestion of BAEE per minute increases the absorption value at 253nm by 0.003 defined as one USP.
QUALITY CONTROL
| Character | white or similar white freeze-dried powder |
Molecular Weight | 23.5KD±2.4KD |
Purity | ≥95%（SDS-PAGE） |
Purity (RP-HPLC) | α-trypsin ≤20% |
β-trypsin≥70% | |
Specific activity | ≥3800USP unit/mg pro |
Host cell DNA residue | ≤100ng/mg pro. |
Host protein residue | ≤100ng/mg pro. |
Optimal pH | 7.0-9.0 |
RECOMMENDED USAGE
Protein preparation - Dissolve the protein in a solution of 25mM Tris HCl at pH 8.0.
Enzyme preparation - Weigh the required enzyme powder and dissolve it in 10mM HCl to achieve an enzyme concentration of 1-10mg/ml.
Reaction conditions - enzyme quantity: target protein (mass ratio)=1:50-1:1000, with an optimal pH of 7.0-9.0
TRANSPORTATION AND STORAGE
Transportation stability: Ice bag insulation transportation, stable activity.
Storage stability: The lyophilized powder can be stored stably for at least 24 months at -15~-20°C. Dissolved with 1 mM hydrochloric acid or 50 mM acetic acid, stored at -15~-20 °C, repeated freeze-thaw 10 times, no loss of activity. It is recommended to store at 2~8 °C for no more than 7 days after dissolution, and no more than 30 days at -15~-20 °C after dissolution.
For Laboratory R&D Use Only.