Leucine Dehydrogenase

CAS：  

9082-71-7

Specific activity:

≥ 25U/mg protein

Unit definition:  

One unit will convert   one micromole of L-Leucine to αKetoisocaproate   per minute at pH 10.5 at 37℃

Catalog No.:

DL31006

Appearance:

White amorphous powder

Source:

Microorganism

Enzyme Commission Number:

EC 1.4.1.9

CAS Number:

9082-71-7

Storage temperature:

-20℃

Specific activity:

≥ 25U/mg protein

Unit definition:

One unit will convert one   micromole of L-Leucine to αKetoisocaproate per minute at pH 10.5 at 37℃

43 kDa (SDS-PAGE)

Isoelectric point:

6.6

Michaelis constant:

2.6×10^-4 M (NAD )

2.0×10^-3 M(L-Leucine)

6.8×10^-4 M(α-Ketoisocaproate)

4.2×10^-2 M (NH Cl) ₄

2.3×10^-4 M (NADH)

Optimum pH:

above11.0(L-Leu→α-K I C)

8.5(α-K I C→ L-Leu )

Optimum temperature:

55-60℃(L-Leu →α-K I C)

> 60℃ (α-K I C→ L-Leu )

pH Stability:

6.0~11.0 (25℃, 15hr)

Thermal stability:

< 55℃ (pH 7.0, 20min)

Inhibitors:

Fe³⁺, NEM, Proclin, SDS