Creatine amidinohydrolase

CAS：   

37340-58-2

Specific activity:  

≥ 10U/mg protein

Unit definition:   

One unit causes the formation of one micromole of sarcosine from creatine per min at pH 7.5 at   37℃.   

Catalog   No.:

DL500361

Appearance:

White amorphous powder

Source:

Microorganism

Enzyme   Commission Number:

EC 3.5.3.3

CAS   Number:

37340-58-2

Storage   temperature:

-20℃

Specific   activity:

≥ 5U/mg protein

Unit   definition:

One unit causes the formation of   one micromole of sarcosine from creatine per min at pH 7.5 at 37℃.

Molecular   weight:

~43kDa (SDS-PAGE)

Isoelectric   point:

5.9

Michaelis   constant:

6.5×10^-3 M (Creatine)

Optimum   pH:

7.0~9.0

Optimum   temperature:

50℃

pH   Stability:

7.5~10.5 (25℃, 24hr)

Thermal   stability:

< 50℃ (pH 7.5, 30min)

Inhibitors:

Cu²⁺ ,Fe³⁺ ,Zn ²⁺ ,NEM,Proclin,SDS