Glutamate Dehydrogenase

CAS：   

9001-46-1

Specific activity:

≥ 400U/mg protein

Unit definition:  

One unit will convert one micromole of α- ketoglutarate to L-glutamate per min at pH 8.3 at 30℃.

Catalog No.:

DL500140

Appearance:

White amorphous powder

Source:

Microorganism

Enzyme Commission Number:

EC 1.4.1.2

CAS Number:

9001-46-1

Storage temperature:

-20℃

Specific activity:

≥ 400U/mg protein

Unit definition:

One unit will convert one   micromole of α- ketoglutarate to L-glutamate per min at pH 8.3 at 30℃.

Molecular weight:

260 kDa (Gel filtration)

Isoelectric point:

5.6

Michaelis constant:

9.5×10^-3 M (NH ) ₃

5.0×10-3M (α-Ketoglutarate)

8.4×10-5M (NADH)

Optimum pH:

8.5(α-KG→L-Glu)

Optimum temperature:

45℃

pH Stability:

5.0~11.0 (25℃, 20hr)

Thermal stability:

< 60℃ (pH 8.3, 10min)

Inhibitors:

Co²⁺ , Fe³⁺,NEM,Proclin,SDS