3α-hydroxysteroid dehydrogenase

CAS：   

9028-56-2

Specific activity:   

≥ 90U/mg protein

Unit definition:

One unit will oxidate one   micromole of androsterone per min at pH 8.25 at 25℃.

Catalog No.:

DL500171

Appearance:

White amorphous powder

Source:

Microorganism

Enzyme Commission Number:

EC 1.1.1.50

CAS Number:

9028-56-2

Storage temperature:

-20℃

Specific activity:

≥ 90U/mg protein

Unit definition:

One unit will oxidate one   micromole of androsterone per min at pH 8.25 at 25℃.

Molecular weight:

28 kDa (SDS-PAGE)

Isoelectric point:

6.4

Michaelis constant:

3.0×10^-5 M(androsterone)

6.0×10^-6 M(NAD )

Optimum pH:

10.5

Optimum temperature:

60℃

pH Stability:

4.5~10.5(30℃,20hr)

Thermal stability:

< 50℃ (pH 7.2, 20min)

Inhibitors:

Cu²⁺ ,Fe³⁺ ,Zn²⁺ ,NEM,Proclin,SDS