Pyruvate oxidase

CAS：   

9001-96-1

Specific activity:  

≥ 50U/mg protein

Unit definition:

One unit will convert one micromole of pyruvate to acetylphosphate per min at pH 6.7at 37℃.

Catalog No.:

DL500470

Appearance:

Yellowish amorphous powder

Source:

Microorganism

Enzyme Commission Number:

EC 1.2.3.3

CAS Number:

9001-96-1

Storage temperature:

-20℃

Specific activity:

≥ 50U/mg protein

Unit definition:

One unit will convert one micromole of pyruvate to acetylphosphate per min at pH 6.7at 37℃.

Molecular weight:

68 kDa (SDS-PAGE)

Isoelectric point:

5

Michaelis constant:

5.3×10-4 M(Pyruvate, pH 7.0)

Optimum pH:

7.0-7.5

Optimum temperature:

50℃

Ph Stability:

6.0~7.5 (37℃, 60min)

Thermal stability:

< 45℃ (pH 6.5, 30min)

Inhibitors:

Cu²⁺, Ni²⁺, Zn²⁺, Proclin, NaN, SDS, Triton X-100