Purine-nucleoside phosphorylase

CAS：   

9030-21-1

Specific activity:   

≥ 200U/mg protein

Unit definition:   

One unit will cause the phosphorolysis of one micromole of inosine to hypoxanthine and ribose 1-phosphate per min at pH 7.7 at 37℃.

Catalog No.:

DL500040

Appearance:

White amorphous powder

Source:

Microorganism

Enzyme Commission Number:

EC 2.4.2.1

CAS Number:

9030-21-1

Storage temperature:

-20℃

Specific activity:

≥ 200U/mg protein

Unit definition:

One unit will cause the phosphorolysis of one micromole of inosine to hypoxanthine and ribose 1-phosphate per min at pH 7.7 at 37℃.

Molecular weight:

32 kDa (SDS-PAGE)

Isoelectric point:

6

Michaelis constant:

2.2×10^-4 M(Inosine)

Optimum pH:

7.5~ 8.0

Optimum temperature:

60℃

pH Stability:

5.0~10.0(30℃,16hr)

Thermal stability:

< 55℃ (pH 7.7, 30min)

Inhibitors:

Co²⁺ ,Cu²⁺ ,Fe³⁺ ,NEM,Proclin,SDS

Inhibitors:

Cu²⁺