Product Name：   

Diaphorase

CAS：   

9001-68-7

Specific activity:   

≥35U/mg protein

Unit definition:   

One unit will convert one micromole of NADH to NAD per minute at pH 8.0 at 37℃.

Catalog No.:

DL500620

Appearance:

Yellowish amorphous powder

Source:

Microorganism

Enzyme Commission Number:

EC 1.6.5.2

CAS Number:

9001-68-7

Storage temperature:

-20℃

Specific activity:

≥ 35U/mg protein

Unit definition:

One Unit will convert one micromole of NADH to NAD per minute at pH 8.0 at 37℃.

Molecular weight:

58KD

Isoelectric point:

4.2

Michaelis constant:

2.6 ×10⁵M (NADH)

Optimum pH:

7.5-8.5       

Optimum temperature:

60℃

pH Stability:

6.5-9.0 (25℃, 24hr)

Thermal stability:

< 45℃ (pH 8.0,   30min)

Inhibitors:

Co²⁺, Cu²⁺, Fe³⁺, Mn²⁺, Ni²⁺, Zn²⁺, NEM, SDS