Pyruvate Kinase

CAS：   

9001-59-6

Specific activity:   

≥ 300U/mg protein

Unit definition:

One unit will convert one   micromole of phosphoenolpyruvate to pyruvate per min at pH 7.2 at 30℃.

Catalog No.:

DL500250

Appearance:

White amorphous powder

Source:

Microorganism

Enzyme Commission Number:

EC 2.7.1.40

CAS Number:

9001-59-6

Storage temperature:

-20℃

Specific activity:

≥ 300U/mg protein

Unit definition:

One unit will convert one   micromole of phosphoenolpyruvate to pyruvate per min at pH 7.2 at 30℃.

Molecular weight:

68 kDa (SDS-PAGE)

Isoelectric point:

5.2

Michaelis constant:

1.1×10 M^-3 (ADP)

2.2×10^-3 M(PEP)

Optimum pH:

7.5

Optimum temperature:

65℃

pH Stability:

5.0~10.0 (37℃, 20hr)

Thermal stability:

< 60℃ (pH 8.5, 20min)

Inhibitors:

Ca²⁺, Co²⁺, Cu²⁺, Fe³⁺, Mn ²⁺, Ni ²⁺,   NEM, Proclin, SDS