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E. coli Poly(A) Polymerase Substitute for NEB M0276S NEB M0276L
Product Information
| Source | Escherichia coli strains carrying the poly(A) polymerase gene cloned from Escherichia coli |
| Storage Buffer | 20mM Tris-HCl (pH 7.5) 300mM NaCl 1mM EDTA 1 mM DTT 0.1% Triton X-100 50% Glycerol |
| Transportation/ Storage Method | Transportation and storage at 20 ± 5 ℃, in order to avoid repeated freezing and thawing, it is recommended to repack immediately after receiving |
| Enzyme Activity Definition | One unit (1U): The amount of enzyme required to incorporate 1 nmol of AMP into RNA within 10 min at 37 °C. |
| Product application | 1. A poly(A) tail can be added to the 3´ end of RNA for cloning or affinity purification of recombinant protein. 2. Can be used for ATP or Cordycepin 3´ end labeling of RNA. 3. Enhance the translation efficiency of RNA transported to eukaryotic cells. 4. Can detect the deletion of DNase and RNase. |
Product Components
| Components | Cat. No. /Size | |
PLA-DL101-A (100U) | PLA-DL101-B (500U) | |
Poly (A) Polymerase (5U/μl) | PLA-DL101-A1(20μl) | PLA-DL101-B1(100μl) |
10×Poly (A) Polymerase Buffer | PLA-DL101-A2(50μl) | PLA-DL101-B2(500μl) |
ATP(100mM） | PLA-DL101-A3(10μl) | PLA-DL101-B3(50μl) |
Other Notes
(1) This Poly (A) polymerase only uses RNA as a substrate.
(2) EDTA can inhibit the activity of this enzyme. It can be purified by adding EDTA to a final concentration of 10 mM, or directly.
(3) Poly (A) polymerase has biological activity only in the presence of divalent cations such as Mg2+.
(4) The tailing effect of Poly (A) polymerase is affected by factors such as enzyme amount, ATP and reaction time. The length of the A tail can be controlled by adjusting the reaction time according to different experimental requirements.
(5) EDTA inhibits the enzyme activity. If the reaction is stopped, it can be purified by adding EDTA to a final concentration of 10 mM, or directly. This product adds AMP to the 3´ end of RNA with high selectivity and does not add the same length of Poly (A) to all RNA molecules.