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E. coli Poly(A) Polymerase, GMP grade
Product Description
Poly(A) Polymerase catalyzes the template independent addition of AMP from ATP to the 3´ end of RNA.
Applications include:
3' labeling of RNA with ATP or Cordycepin and poly(A) tailing of RNA for cloning or affinity purification
Enhances translation of RNA transferred into eukaryotic cells
Tested for the absence of DNases and RNases
Product Information
| Product Name | E. coli Poly(A) Polymerase, GMP grade |
Product Characters | The solution is clear without suspension, precipitation or impurities |
Source | An E. coli strain that carries the cloned Poly(A) Polymerase gene from E. coli |
Storage Condition | -20±5℃ |
Quality Control
| Activity | ≥5U/μl |
Purity (SEC-HPLC) | ≥95% |
Nonspecific nuclease residue | negative |
Endonuclease residue | negative |
Exonuclease residue | negative |
RNA enzyme residue | negative |
Protease residue | negative |
Heavy metal residue | <10ppm |
Bacterial endotoxin | <10EU/ml |
Host DNA residue | <100pg/mg |
Host protein residue | <50ng/mg |
Nickel salt residue | <10ppm |
Microbial limit | <1CFU/10ml |
pH | 8.0±0.5 |
Product Components
Components | Cat. No. /Size | |
GMP-PLA-DL101-A (5KU) | GMP-PLA-DL101-B (100KU) | |
Poly (A) Polymerase (5U/μl) | GMP-PLA-DL101-A1(1000μl) | GMP-PLA-DL101-B1(20ml) |
10×Poly (A) Polymerase Buffer | GMP-PLA-DL101-A2(2500μl) | GMP-PLA-DL101-B2(50ml) |
ATP(100mM） | GMP-PLA-DL101-A3(500μl) | GMP-PLA-DL101-B3(10ml) |
Other Notes
(1) This Poly (A) polymerase only uses RNA as a substrate.
(2) EDTA can inhibit the activity of this enzyme. It can be purified by adding EDTA to a final concentration of 10 mM, or directly.
(3) Poly (A) polymerase has biological activity only in the presence of divalent cations such as Mg2+.
(4) The tailing effect of Poly (A) polymerase is affected by factors such as enzyme amount, ATP and reaction time. The length of the A tail can be controlled by adjusting the reaction time according to different experimental requirements.
(5) EDTA inhibits the enzyme activity. If the reaction is stopped, it can be purified by adding EDTA to a final concentration of 10 mM, or directly. This product adds AMP to the 3´ end of RNA with high selectivity and does not add the same length of Poly (A) to all RNA molecules.