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mRNA Cap 2'-O-Methyltransferase Substitute for NEB M0366S
Analysis of mRNA capping efficiency using LC-MS
Product Information
| Source | E. coli strain (carrying vaccinia virus mRNA cap structure 2´-O-methyltransferase gene) |
| Storage Buffer | 20mM Tris-HCl, pH 8.0 |
| Transportation/ Storage Method | Transportation and storage at 20 ± 5 ℃, in order to avoid repeated freezing and thawing, it is recommended to repack immediately after receiving |
| Enzyme Activity Definition | One unit (1U): The amount of enzyme required to methylate 10 pmol of 80 nt RNA with m7GpppN cap in 1 hour at 37°C. |
| Product application | Using S-adenosylmethionine (SAM) as the methyl donor, a methyl group was added to the 2'-O of the first nucleotide of the cap structure at the 5' end of the RNA, and cap 0 was methylated to cap 1 |
Product Components
Components | Cat. No. /Size | |
MEH-DL101-B(2500U) | MEH-DL101-C (25kU) | |
mRNA Cap 2´-O-Methyltransferase (50U/μl) | MEH-DL101-B1(50μl) | MEH-DL101-C1(500μl) |
10×Capping Buffer | MEH-DL101-B2(200μl) | VCS -DL101-C2(2ml) |
SAM(32mM) | MEH-DL101-B3(50μl) | MEH-DL101-C3(500μl) |
Other Notes
(1) For the safety of experimenters, please wear lab coats and disposable gloves for operation.
(2) The RNA used in the experiment must be purified and dissolved in RNase-free water before use, and the solution must not contain EDTA and salt.
(3) When configuring the reaction system, RNase Inhibitor can be used to enhance the stability of RNA in the reaction.
(4) It is recommended to heat at 65°C for 5 minutes before the reaction to remove the secondary structure of RNA. If the structure of the 5´ end of the transcript is complex, the time can be extended to 10min.
(5) It is recommended to use RNase Inhibitor to enhance the stability of RNA in the reaction. Add 0.5 μl RNase Inhibitor (eg Murine RNase Inhibitor, RNI-DL001) during the reaction.