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Vaccinia Capping Enzyme Vaccinia Capping System Substitute for NEB M2080S NEB M2081S NEB M2081L
Analysis of mRNA capping efficiency using LC-MS
Product Information
| Source | E. coli carrying the capping enzyme gene of vaccinia virus |
Storage Buffer | 20mM Tris-HCl, pH 8.0 |
Transportation/ Storage Method | Transportation and storage at 20 ± 5 ℃, in order to avoid repeated freezing and thawing, it is recommended to repack immediately after receiving |
Enzyme Activity Definition | One unit (1U): The amount of enzyme required to incorporate 10 pmol (α-32P) GTP into an 80-nucleotide (80nt) transcript in 1 hour at 37°C |
Product application | 1, Capping reaction of pre-mRNA translation in vivo/in vitro. |
Product Components
| Components | Cat. No. / Size | |
VCS-DL101-B(500U) | VCS-DL101-C (5000U) | |
Vaccinia Capping Enzyme (10U/μl) | VCS-DL101-B1(50μl) | VCS-DL101-C1 (500μl) |
10 ×Capping Buffer | VCS-DL101-B2(200μl) | VCS-DL101-C2 (1.5ml) |
GTP(10mM) | VCS-DL101-B3(50μl) | VCS-DL101-C3(500μl) |
SAM(32mM) | VCS-DL101-B4(50μl) | VCS-DL101-C4(500μl) |
Other Notes
1) The RNA used for the capping reaction must be purified and resuspended in RNase-free Water.
2) Heat treatment of RNA before the reaction to remove secondary structure at the 5' end.
3) If the structure of the RNA 5' end is known, the reaction time can be extended to 60min to improve the capping efficiency.
4) In the 5' end labeling reaction, the GTP stock solution should be diluted to 1-3 times the molar concentration of mRNA in the reaction system.
5) It is recommended to use RNase inhibitors to enhance the stability of RNA in the reaction. Add 0.5μl of RNase inhibitor (eg Murine RNase Inhibitor, RNI-DL001) during the reaction.