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BsaI, Restriction endonuclease Bsa I
Effect of Digestion
Fig.1 Add 1 μg plasmid and 1 μl BsaI (1-10 lane was diluted by 2 times gradient, 1 lane as stock solution) in 30 min at 37°C in a total reaction volume of 20 μl.
Digestion for 16 h with No Star Activity
Fig.2 Add 1 μg plasmid and 1 μl BsaI (lane 1-12 was diluted by 2 times gradient, lane 1 as stock solution) in 16 h at 37°C in a total reaction volume of 20 μl.
Product Information
| Source | E.coli strain (carries the Eco31IR gene cloned from E. coli RFL31) |
Restriction site | 5'...GGTCTC(N)1↓...3' |
Transportation/ Storage Method | Transportation and storage at 20 ± 5 ℃, in order to avoid repeated freezing and thawing, it is recommended to repack immediately after receiving |
Enzyme Activity Definition | One unit (1U): The amount of enzyme required to completely digest 1 μg of λDNA Dcm-HindIII fragments within 1 h in a 50 μl reaction system at 37°C. |
Product application | Rapid digestion and linearization of plasmid DNA, PCR products or genomic DNA |
Product Components
Components | Cat. No. / Size | |
BSA-DL101-B (1000U) | BSA-DL101-C (5000U) | |
BsaI (20U/μl) | BSA-DL101-B1(50μl) | BSA-DL101-C1(250μl) |
10 ×Reaction Buffer | BSA-DL101-B2(250μl) | BSA-DL101-C2(1ml) |
Other Notes
1. The methylation modification of Dcm, CpG and EcoB I will damage the cleavage activity of this product.
2. Please wear lab clothes and disposable gloves during operation.