- Detail
- Parameters
- Review
DNase I (RNase-free) DNase I RNase-free Deoxyribonuclease I Substitute for NEB M0303S NEB M0303L Promega Z3585 Thermo EN0521
DNase I digests DNA efficiently
Fig.1 Add 1 μg DNA and different activity units of DNaseI in a total reaction volume of 10 μl. Compared with competitor 1 and 2, DNase I of kactus has a stronger digestive effect.
Product Information
| Source | Recombinant enzyme, from a non-animal host |
Storage Buffer | 10mM Tris-HCl (pH7.6) |
Transportation/ Storage Method | Shipped on dry ice, stored at -20°C, valid for one year, in order to avoid repeated freezing and thawing, it is recommended to repack immediately after receiving |
Enzyme Activity Definition | One unit (1U): The amount of enzyme required to completely degrade 1 μg of pBR322 plasmid DNA within 10 minutes at 37°C. |
Product application | 1. Preparation of DNA-free RNA samples. |
Product Components
Product Components | Cat. No. /Size | |
DNI-DL001-B (1000U） | DNI-DL001-C (5000U) | |
DNase I (RNase-free, 4U/μl) | 250μl | 1.25ml |
Other Notes
1. Put this product on ice for operation, and store it at -20°C after use.
2. In RNA extraction experiments, it is recommended to add an appropriate amount of RNase Inhibitor to prevent RNA degradation.
3. Metal ion chelators, 0.1% SDS, DTT and mercaptoethanol have significant inhibitory effects on this product.
4.Please wear lab clothes and disposable gloves during operation.