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Pyrophosphatase, Inorganic
Inorganic pyrophosphatase effectively increases the yield of mRNA
Fig. 1 The addition of inorganic pyrophosphatase to IVT reactions can effectively increase mRNA yield, lane 1 was without inorganic pyrophosphatase, while lane 2 was added inorganic pyrophosphatase.
Product Information
Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to form orthophosphate.
A variety of metabolic reactions generate inorganic pyrophosphate as a reaction byproduct. Such reactions are rendered irreversible when the pyrophosphate is degraded by pyrophosphatase (1). RNA and DNA synthesis are examples of reactions that can be pulled far in the synthesis direction by the action of inorganic pyrophosphatase.
| Source | Recombinant E.coli |
Storage Buffer | 20mM Tris-HCl, pH 8.0 |
Transportation/ Storage Method | Dry ice transportation, stored at -20 ± 5 ° C, valid for one year |
Enzyme Activity Definition | One unit is the amount of enzyme that will generate 1 µmol of phosphate per minute from inorganic pyrophosphate under standard reaction conditions (a 10 minute reaction at 25°C in 100 mM Tris-HCl, [pH 7.2], 2 mM MgCl2 and 2 mM PPi in a reaction volume of 0.5 ml). |
Product application | 1, Optimize RNA transcription and improve RNA production in vitro transcription reaction; |
Product Components
| Product Components | Cat No / Size | |
DL-EE201-A(10U) | DL-EE201-B(50U) | |
Pyrophosphatase, Inorganic(0.1U/μl) | 100μl | 500μl |
Other Notes
(1) This product is active in various reaction buffers. Usually, this product can be directly added in experiments such as HDA amplification and LAMP amplification.
(2) The optimum reaction temperature of this product is 25°C, it is active at 16°C-37°C, and the enzyme can be inactivated at 65°C for 10 minutes.
(3) It is recommended to store in aliquots to avoid repeated freezing and thawing.
(4) Please wear lab clothes and disposable gloves during operation.